4ZFF

Dual-acting Fab 5A12 in complex with VEGF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97856
Spacegroup name	P 21 21 2
Unit cell lengths	87.406, 313.892, 51.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.950 - 2.750
R-factor	0.1994
Rwork	0.197
R-free	0.23770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.170
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.850
High resolution limit [Å]	2.750	5.920	2.750
Rmerge		0.065	0.584
Number of reflections	38026
<I/σ(I)>	16.492
Completeness [%]	99.8	98.6	100
Redundancy	6	5.6	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	292	0.1 M Tris pH 8.0, 2.0 M ammonium sulfate, 0.3 M NDSB-195