4ZF5
Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(Cl2Y), H148D; circular permutant ( 50-51)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.140, 68.130, 58.000 |
| Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
| Resolution | 56.662 - 1.700 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.23240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2duf |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.250 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 56.700 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.079 |
| Number of reflections | 38429 |
| <I/σ(I)> | 13.4 |
| Completeness [%] | 99.4 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 50 mM sodium acetate, 100 mM NaCl, 5% (wt/vol) PEG 3550 |
