4ZF5

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(Cl2Y), H148D; circular permutant ( 50-51)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Green fluorescent protein	polymer	252	28260.4	2	UniProt (P42212)	Aequorea victoria (Jellyfish)
2	C, D (A, B)	water	water		18.0	58	Chemie (HOH)

Sequence modifications

A, B: 1 - 187 (UniProt: P42212)

PDB	External Database	Details
Met -10	-	expression tag
Gly -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Ser -2	-	expression tag
Ser -1	-	expression tag
Gly 0	-	expression tag
Leu 14	Phe 64	engineered mutation
4nu 15	Ser 65	chromophore
4nu	Tyr 66	chromophore
4nu	Gly 67	chromophore
Arg 30	Gln 80	engineered mutation
Ser 49	Phe 99	engineered mutation
Lys 55	Asn 105	engineered mutation
Val 61	Glu 111	engineered mutation
Thr 78	Ile 128	engineered mutation
Phe 95	Tyr 145	engineered mutation
Asp 98	His 148	engineered mutation
Thr 103	Met 153	engineered mutation
Ala 113	Val 163	engineered mutation
Thr 116	Lys 166	engineered mutation
Val 117	Ile 167	engineered mutation
Val 121	Ile 171	engineered mutation
Thr 155	Ser 205	engineered mutation
Val 156	Ala 206	engineered mutation

A, B: 197 - 243 (UniProt: P42212)

PDB	External Database	Details
Gly 188	-	linker
Gly 189	-	linker
Thr 190	-	linker
Gly 191	-	linker
Gly 192	-	linker
Ser 193	-	linker
Ala 194	-	linker
Ser 195	-	linker
Gln 196	-	linker
Arg 223	Ser 30	engineered mutation
Ile 232	Tyr 39	engineered mutation
Ser 241	Cys 48	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56520.7
All*	Total formula weight	56520.7

*Water molecules are not included.