crystal structure of a green fluorescent protein variant S65T/H148D at pH 5.6

Summary for 2DUF

Related2DUE 2DUG 2DUH 2DUI
DescriptorGreen fluorescent protein (2 entities in total)
Functional Keywordsexcited state proton transfer, very short hydrogen bond, green fluorescent protein, luminescent protein
Biological sourceAequorea victoria
Total number of polymer chains1
Total molecular weight26907.33
Shu, X.,Remington, S.J. (deposition date: 2006-07-23, release date: 2007-07-10, Last modification date: 2011-07-13)
Primary citation
Shu, X.,Kallio, K.,Shi, X.,Abbyad, P.,Kanchanawong, P.,Childs, W.,Boxer, S.G.,Remington, S.J.
Ultrafast excited-state dynamics in the green fluorescent protein variant S65T/H148D. 1. Mutagenesis and structural studies.
Biochemistry, 46:12005-12013, 2007
PubMed: 17918959 (PDB entries with the same primary citation)
DOI: 10.1021/bi7009037
MImport into Mendeley
Experimental method
NMR Information

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.1781304.9%4.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

Molmil generated image of 2duf
no rotation
Molmil generated image of 2duf
rotated about x axis by 90°
Molmil generated image of 2duf
rotated about y axis by 90°