2DUF

crystal structure of a green fluorescent protein variant S65T/H148D at pH 5.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	51.106, 63.025, 68.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.500
R-factor	0.172
Rwork	0.170
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	34193
Completeness [%]	94.7	68.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	100mM MgCl2, 100mM NaOAc pH 5.6, 26% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 298K