4ZF4

Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(Cl1Y), H148D; circular permutant (50-51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.10
Spacegroup name	P 1 21 1
Unit cell lengths	51.840, 68.590, 60.880
Unit cell angles	90.00, 100.50, 90.00

Refinement procedure

Resolution	36.344 - 1.823
R-factor	0.2007
Rwork	0.198
R-free	0.24580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2duf
RMSD bond length	0.007
RMSD bond angle	1.064
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.344
High resolution limit [Å]	1.800
Rmerge	0.107
Number of reflections	37043
<I/σ(I)>	6.7
Completeness [%]	98.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	50 mM sodium acetate, 100 mM NaCl, 15% (wt/vol) PEG 3550