4ZF4
Crystal structure of Green Fluorescent Protein (GFP); S65T, Y66(Cl1Y), H148D; circular permutant (50-51)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Green fluorescent protein | polymer | 252 | 28225.9 | 2 | UniProt (P42212) In PDB | Aequorea victoria (Jellyfish) | |
2 | water | water | 18.0 | 99 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 187 (UniProt: P42212)
A, B: 197 - 243 (UniProt: P42212)
PDB | External Database | Details |
---|---|---|
Met -10 | - | initiating methionine |
Gly -9 | - | expression tag |
His -8 | - | expression tag |
His -7 | - | expression tag |
His -6 | - | expression tag |
His -5 | - | expression tag |
His -4 | - | expression tag |
His -3 | - | expression tag |
Ser -2 | - | expression tag |
Ser -1 | - | expression tag |
Gly 0 | - | expression tag |
Leu 14 | Phe 64 | engineered mutation |
4nt 15 | Ser 65 | chromophore |
4nt | Tyr 66 | chromophore |
4nt | Gly 67 | chromophore |
Arg 30 | Gln 80 | engineered mutation |
Ser 49 | Phe 99 | engineered mutation |
Lys 55 | Asn 105 | engineered mutation |
Val 61 | Glu 111 | engineered mutation |
Thr 78 | Ile 128 | engineered mutation |
Phe 95 | Tyr 145 | engineered mutation |
Asp 98 | His 148 | engineered mutation |
Thr 103 | Met 153 | engineered mutation |
Ala 113 | Val 163 | engineered mutation |
Thr 116 | Lys 166 | engineered mutation |
Val 117 | Ile 167 | engineered mutation |
Val 121 | Ile 171 | engineered mutation |
Thr 155 | Ser 205 | engineered mutation |
Val 156 | Ala 206 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 188 | - | linker |
Gly 189 | - | linker |
Thr 190 | - | linker |
Gly 191 | - | linker |
Gly 192 | - | linker |
Ser 193 | - | linker |
Ala 194 | - | linker |
Ser 195 | - | linker |
Gln 196 | - | linker |
Arg 223 | Ser 30 | engineered mutation |
Ile 232 | Tyr 39 | engineered mutation |
Ser 241 | Cys 48 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 56451.8 | |
All* | Total formula weight | 56451.8 |