4ZER

Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	209.300, 452.290, 625.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.723 - 3.100
R-factor	0.2329
Rwork	0.231
R-free	0.27130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.015
RMSD bond angle	0.809
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.220	0.064	1.423
Rmeas	0.255	0.074	1.664
Total number of observations	3999403
Number of reflections	1050694	11690	77154
<I/σ(I)>	5.47	19.06	0.95
Completeness [%]	99.1	92.5	98.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	Tris-HCl, PEG 20000, MPD, Arginine