4ZER
Crystal structure of the Onc112 antimicrobial peptide bound to the Thermus thermophilus 70S ribosome
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.300, 452.290, 625.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.723 - 3.100 |
| R-factor | 0.2329 |
| Rwork | 0.231 |
| R-free | 0.27130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y4o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.809 |
| Data scaling software | XSCALE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 3.180 | ||
| High resolution limit [Å] | 3.100 | 13.860 | 3.100 |
| Rmerge | 0.220 | 0.064 | 1.423 |
| Rmeas | 0.255 | 0.074 | 1.664 |
| Total number of observations | 3999403 | ||
| Number of reflections | 1050694 | 11690 | 77154 |
| <I/σ(I)> | 5.47 | 19.06 | 0.95 |
| Completeness [%] | 99.1 | 92.5 | 98.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 293 | Tris-HCl, PEG 20000, MPD, Arginine |
