4ZDW
Crystal structure of the Rab GTPase Sec4p mutant - S29V in complex with Sec2p and GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.08090 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 116.900, 119.280, 122.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 85.440 - 2.900 |
| R-factor | 0.25993 |
| Rwork | 0.258 |
| R-free | 0.29527 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2eqb |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.613 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.440 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.084 | 0.510 |
| Number of reflections | 19416 | |
| <I/σ(I)> | 13.4 | 3.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.9 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Crystals were grown by mixing protein complex with a reservoir solution containing 24% PEG 3350, 0.2 M Sodium Citrate in a 1:1 ratio and with the addition of 10mM GDP to the drop. |
