4ZDO
The crystal structure of T325S mutant of human SepSecS in complex with selenocysteine tRNA (tRNASec)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-02-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 166.238, 166.238, 238.160 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.492 - 2.650 |
R-factor | 0.1996 |
Rwork | 0.198 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hl2 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.044 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 6.510 | 2.400 |
Rmerge | 0.152 | 0.060 | |
Rmeas | 0.161 | 0.063 | |
Rpim | 0.059 | 0.019 | |
Total number of observations | 1085896 | ||
Number of reflections | 133611 | ||
<I/σ(I)> | 5 | ||
Completeness [%] | 90.9 | 99.9 | 35 |
Redundancy | 8.1 | 11 | 1.5 |
CC(1/2) | 0.995 | 0.030 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1M Na-cacodylate, pH 6.4 0.24M lithium citrate 10-12% (w/v) PEG 3,350 |