4ZDA
Crystal structure of isocitrate dehydrogenase in complex with isocitrate and Mn from M. smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-24 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.774, 206.162, 145.598 |
| Unit cell angles | 90.00, 90.97, 90.00 |
Refinement procedure
| Resolution | 48.590 - 2.800 |
| R-factor | 0.21611 |
| Rwork | 0.214 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.033 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.800 |
| Number of reflections | 145405 |
| <I/σ(I)> | 12.81 |
| Completeness [%] | 97.4 |
| Redundancy | 2.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05 M MgCl2, 28-34% Polyethylene glycol monomethyl ether 550 (Qiagen), and 0.1M Hepes pH 7.5 |
