4ZCH
Single-chain human APRIL-BAFF-BAFF Heterotrimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.041, 117.865, 295.521 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.610 - 2.430 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1Q5X and 1KD7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.760 | 2.680 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.079 | 0.505 |
| Number of reflections | 36901 | |
| <I/σ(I)> | 13.46 | |
| Completeness [%] | 96.7 | 97.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | RESERVOIR SOLUTION : 14% PEG6000 , 1M LiCL , TRIS-HCL pH 8.50 |
