4ZC7
Paromomycin bound to a leishmanial ribosomal A-site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-28 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9394 |
Spacegroup name | P 1 |
Unit cell lengths | 31.690, 57.180, 47.700 |
Unit cell angles | 78.92, 109.44, 123.61 |
Refinement procedure
Resolution | 44.979 - 3.041 |
R-factor | 0.1912 |
Rwork | 0.183 |
R-free | 0.26140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j7t |
RMSD bond length | 0.006 |
RMSD bond angle | 1.230 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.000 | 45.000 | 3.120 |
High resolution limit [Å] | 3.040 | 13.600 | 3.040 |
Rmerge | 0.090 | 0.033 | 0.283 |
Rmeas | 0.106 | 0.040 | 0.339 |
Total number of observations | 15718 | ||
Number of reflections | 5004 | 46 | 382 |
<I/σ(I)> | 7.05 | 14.46 | 2.13 |
Completeness [%] | 98.5 | 80.7 | 100 |
Redundancy | 3.1 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | Sodium cacodylate pH 7.0, spermine tetrahydrochloride, 2-methyl-2,4-pentanediol (MPD), MgSO4, KCl. |