4Z95

Fab structure of antibody S1-15 in complex with ssDNA DNA, 5'-5(dT)-p-3'

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	113
Detector technology	CCD
Collection date	2014-06-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 43 21 2
Unit cell lengths	77.761, 77.761, 156.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.790
R-factor	0.2045
Rwork	0.203
R-free	0.23620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4odt
RMSD bond length	0.010
RMSD bond angle	1.336
Data reduction software	HKL-2000 (v701c3)
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.850
High resolution limit [Å]	1.790	1.790
Rmerge		0.740
Number of reflections	43749
<I/σ(I)>	37.7	3.7
Completeness [%]	99.9	100
Redundancy	9.9	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289.15	0.1M Tris-HCl pH 8.5, 25% (v/v) PEG 550 MME