4Z7M
Novel Inhibitors of Bacterial Methionine Aminopeptidase with Broad-Spectrum Biochemical Activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-27 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.985, 62.712, 72.978 |
| Unit cell angles | 90.00, 89.59, 90.00 |
Refinement procedure
| Resolution | 47.570 - 1.430 |
| R-factor | 0.2539 |
| Rwork | 0.251 |
| R-free | 0.30550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.863 |
| Data reduction software | d*TREK |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.570 |
| High resolution limit [Å] | 1.430 |
| Number of reflections | 71302 |
| <I/σ(I)> | 4.3 |
| Completeness [%] | 92.1 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 100 mM MES, 1.1 M ammonium sulfate, 0.1 M potassium chloride, 9% trehalose |
