4Z30

Crystal structure of the ROQ domain of human Roquin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-02-19
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 43 21 2
Unit cell lengths	144.764, 144.764, 56.141
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.710
R-factor	0.2218
Rwork	0.220
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qik
RMSD bond length	0.010
RMSD bond angle	1.409
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.760
High resolution limit [Å]	2.710	7.350	2.710
Rmerge	0.083	0.039	0.905
Rmeas	0.088	0.041	0.956
Rpim	0.028	0.014	0.302
Total number of observations	160955
Number of reflections	16736
<I/σ(I)>	9.8
Completeness [%]	100.0	100	99.9
Redundancy	9.6	8.5	9.7
CC(1/2)		0.999	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	298	mixing 2 uL protein with 1 uL well solution consisting of 3.2 M NaCl, 0.1 M NaOAc pH4.6