4Z1D
Structure of PEP and zinc bound KDO8PS from H.pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-20 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.300, 51.013, 78.547 |
| Unit cell angles | 90.00, 104.37, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.18706 |
| Rwork | 0.186 |
| R-free | 0.21210 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.185 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.650 |
| Number of reflections | 65566 |
| <I/σ(I)> | 34.31 |
| Completeness [%] | 99.2 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 3350, HEPES, magnesium chloride |
