4Z0I
Crystal structure of a tetramer of GluA2 ligand binding domains bound with glutamate at 1.45 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 47.190, 47.270, 116.820 |
| Unit cell angles | 90.00, 93.57, 90.00 |
Refinement procedure
| Resolution | 47.270 - 1.450 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ftj |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.190 |
| Data reduction software | MOSFLM |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 90541 | |
| <I/σ(I)> | 11.7 | 2.6 |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277.14 | 20% PEG 3350 and 200 mM KNO3 |
