4YUV
Crystal structure of Trypanosoma cruzi spermidine synthase in complex with decarboxylated S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2013-12-16 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.354, 92.364, 68.269 |
| Unit cell angles | 90.00, 99.76, 90.00 |
Refinement procedure
| Resolution | 24.710 - 1.600 |
| R-factor | 0.19781 |
| Rwork | 0.196 |
| R-free | 0.24012 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bwb |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.292 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.710 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.157 |
| Number of reflections | 65545 | |
| <I/σ(I)> | 14 | 3.4 |
| Completeness [%] | 96.8 | 99.9 |
| Redundancy | 3.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG4000, ammonium sulfate, bis-Tris |
