4YU9
Crystal Structure of double mutant Y115E Y117E human Glutaminyl Cyclase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-28 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.920 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.380, 149.630, 96.300 |
| Unit cell angles | 90.00, 96.68, 90.00 |
Refinement procedure
| Resolution | 95.650 - 2.100 |
| R-factor | 0.213 |
| Rwork | 0.211 |
| R-free | 0.25420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.771 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 95.700 |
| High resolution limit [Å] | 2.100 |
| Rmerge | 0.143 |
| Number of reflections | 66975 |
| <I/σ(I)> | 5.1 |
| Completeness [%] | 95.0 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 281 | 0.1 M MES pH 6.5, 1.6 M Ammonium Sulfate |
