4YQX

Mouse IL-2 Bound to JES6-1 scFv Fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-01-14
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976962
Spacegroup name	P 31 2 1
Unit cell lengths	99.024, 99.024, 198.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.512 - 2.826
R-factor	0.1712
Rwork	0.170
R-free	0.20430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b5i
RMSD bond length	0.005
RMSD bond angle	0.857
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1951))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.920
High resolution limit [Å]	2.820	6.070	2.820
Rmerge	0.115	0.050	0.679
Rmeas	0.121	0.053	0.728
Rpim	0.038	0.017	0.257
Total number of observations	268758
Number of reflections	27586
<I/σ(I)>	7.7
Completeness [%]	99.9	100	99.3
Redundancy	9.7	9.6	7.4
CC(1/2)		0.999	0.842

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.4	295	0.1 M Bis-Tris propane, pH 6.4, 0.2 M sodium citrate, 19% PEG3350