4YPM
Crystal structure of a LonA protease domain in complex with bortezomib
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2014-03-18 |
| Detector | RAYONIX MX325HE |
| Wavelength(s) | 0.8 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 122.343, 122.343, 103.125 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.850 - 1.850 |
| R-factor | 0.17835 |
| Rwork | 0.177 |
| R-free | 0.20094 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.386 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.110 | |
| Number of reflections | 39225 | |
| <I/σ(I)> | 33.44 | 4.629 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 6.5 | 295 | 0.2 M sodium citrate pH 6.5 and 10 % PEG 3350 |
