4YPM

Crystal structure of a LonA protease domain in complex with bortezomib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL15A1
Synchrotron site	NSRRC
Beamline	BL15A1
Temperature [K]	200
Detector technology	CCD
Collection date	2014-03-18
Detector	RAYONIX MX325HE
Wavelength(s)	0.8
Spacegroup name	P 6 2 2
Unit cell lengths	122.343, 122.343, 103.125
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.850 - 1.850
R-factor	0.17835
Rwork	0.177
R-free	0.20094
RMSD bond length	0.009
RMSD bond angle	1.386
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.110
Number of reflections	39225
<I/σ(I)>	33.44	4.629
Completeness [%]	100.0	100
Redundancy	21.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	295	0.2 M sodium citrate pH 6.5 and 10 % PEG 3350