4YPG
Structural Insights Into the Neutralization Properties of a Human Anti-Interferon Monoclonal Antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 134.820, 153.260, 163.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.20942 |
| Rwork | 0.206 |
| R-free | 0.27166 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rh2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.377 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.120 | 0.420 |
| Number of reflections | 34084 | |
| <I/σ(I)> | 7.8 | 2.7 |
| Completeness [%] | 99.8 | 99.4 |
| Redundancy | 4.6 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.01 M NiCl2, 0.1 M Tris HCl |
