4YOQ
Crystal Structure of MutY bound to its anti-substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.697, 84.594, 142.457 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.408 - 2.210 |
R-factor | 0.1876 |
Rwork | 0.184 |
R-free | 0.24960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rrq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.399 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 142.460 | 2.280 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 1.523 | |
Number of reflections | 23671 | |
<I/σ(I)> | 11.3 | 1.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100 mM Tris, pH 8.0, 250 mM NaOAc, 29% (wt/vol) PEG 4000 |