4YOL

Human fibroblast growth factor-1 C16S/A66C/C117A/P134A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	75.630, 97.831, 107.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.815 - 1.970
R-factor	0.1625
Rwork	0.160
R-free	0.19930
RMSD bond length	0.007
RMSD bond angle	1.060
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.970	1.970
Rmerge		0.405
Number of reflections	28191
<I/σ(I)>	39	9
Completeness [%]	97.7	99.2
Redundancy	9.7	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.7 M sodium citrate, 0.1 M imidazole, pH 8.5