4YJV

THE KINASE DOMAIN OF HUMAN SPLEEN TYROSINE (SYK) IN COMPLEX WITH GTC000250

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	OTHER
Temperature [K]	100
Detector technology	CCD
Collection date	2005-08-15
Detector	MAR CCD 130 mm
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	39.460, 86.940, 40.430
Unit cell angles	90.00, 92.59, 90.00

Refinement procedure

Resolution	27.590 - 1.652
R-factor	0.1773
Rwork	0.175
R-free	0.22230
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	In-house SYK model
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	MOSFLM (6.2.4)
Data scaling software	SCALA (3.2.5)
Refinement software	BUSTER (2.11.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.597	27.597	1.740
High resolution limit [Å]	1.652	5.220	1.652
Rmerge	0.035	0.035	0.122
Total number of observations	68929	2254	8358
Number of reflections	30428
<I/σ(I)>	22.8	36.4	7.8
Completeness [%]	93.1	94	83.3
Redundancy	2.3	2.2	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	30% Jeffamine ED-2001, 10% Glycerol, 0.1M MES pH6.0, 5mM TCEP