4YIV
Crystal structure of engineered TgAMA1 lacking the DII loop
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 89.050, 89.050, 124.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.824 - 1.930 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.19510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y8t |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.356 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.240 | 2.110 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.081 | 0.494 |
| Number of reflections | 38493 | |
| <I/σ(I)> | 13.8 | 4 |
| Completeness [%] | 99.8 | 91.9 |
| Redundancy | 7.7 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 5 mM cobalt (II) chloride hexahydrate, 5 mM nickel (II) chloride hexahydrate, 5 mM cadmium chloride hydrate, 5 mM magnesium chloride hexahydrate, 0.1 M Hepes pH 7.5, 12% PEG 3350, 2% glycerol |
