4YHH
Crystal structure of the 30S ribosomal subunit from Thermus thermophilus in complex with tigecycline
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-02-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 409.590, 409.590, 171.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.716 - 3.417 |
| R-factor | 0.2312 |
| Rwork | 0.228 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zm6 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS (July 4, 2012) |
| Data scaling software | XSCALE (July 4, 2012) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.580 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 6.995 | |
| Number of reflections | 192315 | |
| <I/σ(I)> | 4.45 | 0.3 |
| Completeness [%] | 96.7 | 84.3 |
| Redundancy | 4.5 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | HEPES-KOH, MgCl2, NH4Cl, beta-mercaptoethanol, MPD, spermidine |
