4YE2
The 1.35 structure of a viral RNase L antagonist reveals basis for the 2'-5'-oligoadenylate binding and enzyme activity.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.976484 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 97.137, 97.137, 110.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.503 - 3.098 |
| R-factor | 0.2611 |
| Rwork | 0.261 |
| R-free | 0.27240 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rpt |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.141 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1839)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 3.150 |
| High resolution limit [Å] | 3.098 | 3.100 |
| Rmerge | 0.146 | 0.800 |
| Number of reflections | 10030 | |
| <I/σ(I)> | 23.6 | 4.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 18.4 | 18.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M HEPES sodium, 2% PEG400, 2.0M Ammonium sulfate |
