4YDH

The structure of human FMNL1 N-terminal domains bound to Cdc42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-09-25
Detector	MARRESEARCH
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	87.600, 102.100, 170.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.698 - 3.800
R-factor	0.2114
Rwork	0.208
R-free	0.28090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4yc7
RMSD bond length	0.005
RMSD bond angle	1.176
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	3.800	3.800
Rmerge	0.186
Number of reflections	15654
<I/σ(I)>	11.8
Completeness [%]	99.4
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	17% (v/v) PEG 3350, 0.16 M tri-ammonium citrate