4Y6O

Human SIRT2 in complex with myristoylated peptide (TNF-alphaK20myr)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	37.136, 48.785, 96.960
Unit cell angles	101.07, 91.33, 112.27

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.20769
Rwork	0.206
R-free	0.23457
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1j8f 3zgo
RMSD bond length	0.007
RMSD bond angle	1.215
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.600
Number of reflections	77514
<I/σ(I)>	14.3
Completeness [%]	95.7
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG 3350