4Y3O
Crystal structure of Ribosomal oxygenase NO66 in complex with substrate Rpl8 peptide and Ni(II) and cofactor N-oxalyglycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-09-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.500, 202.930, 85.680 |
| Unit cell angles | 90.00, 118.94, 90.00 |
Refinement procedure
| Resolution | 40.570 - 2.200 |
| R-factor | 0.20578 |
| Rwork | 0.203 |
| R-free | 0.26154 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.922 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.123 | 0.502 |
| Number of reflections | 62029 | |
| <I/σ(I)> | 7.6 | 2.4 |
| Completeness [%] | 98.0 | 97.8 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1 M Imidazole pH 6.5, 0.5 M Sodium acetate trihydrate |
