4Y3C
I304V 3D polymerase mutant of EMCV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-27 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 232.332, 140.775, 171.838 |
| Unit cell angles | 90.00, 126.03, 90.00 |
Refinement procedure
| Resolution | 138.960 - 3.200 |
| R-factor | 0.22154 |
| Rwork | 0.220 |
| R-free | 0.24983 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nz0 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.773 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.137 | 0.469 |
| Number of reflections | 73423 | |
| <I/σ(I)> | 5.2 | 2.2 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 2.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 4.0M AMMONIUM ACETATE, 0.1M SODIUM ACETATE PH 4.6 |
