4Y30

Crystal structure of human protein arginine methyltransferase PRMT6 bound to SAH and EPZ020411

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 43
Unit cell lengths	99.672, 99.672, 89.252
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.510 - 2.100
R-factor	0.1818
Rwork	0.180
R-free	0.22080
RMSD bond length	0.010
RMSD bond angle	1.386
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	99.670
High resolution limit [Å]	2.100
Number of reflections	50960
<I/σ(I)>	8.3
Completeness [%]	99.8
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	MgCl, MES buffer pH 6.5, Isopropanol, PEG 4000