4Y0F
Crystal Structure of Human TDP-43 RRM1 Domain in Complex with an Unmodified Single-stranded DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2011-02-17 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 100.027, 100.027, 98.144 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.701 - 2.648 |
R-factor | 0.2451 |
Rwork | 0.240 |
R-free | 0.28830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4iuf |
RMSD bond length | 0.002 |
RMSD bond angle | 0.664 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 27.701 |
High resolution limit [Å] | 2.648 |
Number of reflections | 7991 |
<I/σ(I)> | 44.43 |
Completeness [%] | 93.6 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.12M ammonium acetate, 0.08M BIS-TRIS, pH 5.5, 20% PEG 3350 |