4Y0F

Crystal Structure of Human TDP-43 RRM1 Domain in Complex with an Unmodified Single-stranded DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	110
Detector technology	CCD
Collection date	2011-02-17
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	P 65 2 2
Unit cell lengths	100.027, 100.027, 98.144
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.701 - 2.648
R-factor	0.2451
Rwork	0.240
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iuf
RMSD bond length	0.002
RMSD bond angle	0.664
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	27.701
High resolution limit [Å]	2.648
Number of reflections	7991
<I/σ(I)>	44.43
Completeness [%]	93.6
Redundancy	9.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.12M ammonium acetate, 0.08M BIS-TRIS, pH 5.5, 20% PEG 3350