4Y00
Crystal Structure of Human TDP-43 RRM1 Domain with D169G Mutation in Complex with an Unmodified Single-stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2014-01-04 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 97.636, 97.636, 96.871 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 16.847 - 3.000 |
| R-factor | 0.2746 |
| Rwork | 0.266 |
| R-free | 0.29490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iuf |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.618 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.850 | 3.003 |
| High resolution limit [Å] | 3.000 | 2.899 |
| Rmerge | 0.061 | 0.508 |
| Number of reflections | 10669 | |
| <I/σ(I)> | 18.05 | 5.96 |
| Completeness [%] | 97.9 | 100 |
| Redundancy | 6.2 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.05 M CH3COONH4, pH 5.0, 15% v/v Jeffamine ED-2001, pH 7.0 |
