4Y00
Crystal Structure of Human TDP-43 RRM1 Domain with D169G Mutation in Complex with an Unmodified Single-stranded DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2014-01-04 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 97.636, 97.636, 96.871 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 16.847 - 3.000 |
R-factor | 0.2746 |
Rwork | 0.266 |
R-free | 0.29490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4iuf |
RMSD bond length | 0.003 |
RMSD bond angle | 0.618 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 16.850 | 3.003 |
High resolution limit [Å] | 3.000 | 2.899 |
Rmerge | 0.061 | 0.508 |
Number of reflections | 10669 | |
<I/σ(I)> | 18.05 | 5.96 |
Completeness [%] | 97.9 | 100 |
Redundancy | 6.2 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.05 M CH3COONH4, pH 5.0, 15% v/v Jeffamine ED-2001, pH 7.0 |