4XXD
Crystal Structure of mid-region amyloid beta capture by solanezumab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.796, 73.555, 92.118 |
| Unit cell angles | 109.91, 93.64, 93.31 |
Refinement procedure
| Resolution | 46.560 - 2.410 |
| R-factor | 0.2512 |
| Rwork | 0.249 |
| R-free | 0.28980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hix |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.290 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.560 | 2.510 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.117 | 0.543 |
| Number of reflections | 35968 | |
| <I/σ(I)> | 8.2 | 2.3 |
| Completeness [%] | 97.9 | 91.9 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 292 | PEG 3350, sodium citrate |
