4XUS
Crystal structure of the pre-catalytic ternary complex of DNA polymerase lambda with a templating A and an incoming dTTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.050, 63.280, 141.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.960 - 2.400 |
R-factor | 0.1898 |
Rwork | 0.187 |
R-free | 0.23580 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | Aimless |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.717 | |
Number of reflections | 17565 | |
<I/σ(I)> | 13.4 | 3.1 |
Completeness [%] | 86.0 | 44 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.1 M sodium acetate trihydrate 2.0 M sodium formate |