4XNZ
Crystal structure of broadly and potently neutralizing antibody VRC06B in complex with HIV-1 clade A/E strain 93TH057 gp120
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-12 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.314, 189.442, 219.935 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.895 - 3.389 |
| R-factor | 0.2469 |
| Rwork | 0.244 |
| R-free | 0.30110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jb9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.558 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.460 |
| High resolution limit [Å] | 3.389 | 9.210 | 3.389 |
| Rmerge | 0.099 | 0.054 | 0.561 |
| Rmeas | 0.108 | 0.059 | 0.640 |
| Rpim | 0.042 | 0.024 | 0.297 |
| Total number of observations | 217360 | ||
| Number of reflections | 35035 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 87.8 | 97.2 | 51 |
| Redundancy | 6.2 | 6 | 3.6 |
| CC(1/2) | 0.997 | 0.714 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 7% PEG 3350, 2.5% isopropanol, 0.1M Tris-HCl, pH 8.5 |
