4XNW
The human P2Y1 receptor in complex with MRS2500
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 40.670, 64.970, 77.570 |
Unit cell angles | 101.82, 95.70, 100.85 |
Refinement procedure
Resolution | 29.310 - 2.700 |
R-factor | 0.22094 |
Rwork | 0.218 |
R-free | 0.26714 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.331 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 20471 |
<I/σ(I)> | 18.9 |
Completeness [%] | 98.2 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 293 | PEG400 Sodium citrate HEPES |