4XLV
Crystal structure of the activated insulin receptor tyrosine kinase dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-04-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 66.982, 66.982, 136.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.20728 |
| Rwork | 0.204 |
| R-free | 0.26625 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.482 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 16312 |
| <I/σ(I)> | 17.1 |
| Completeness [%] | 98.9 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM Tris-HCl, pH 7.5, 26% (w/v) polyethylene glycol (PEG) 4000, 200 mM MgCl2, and 5 mM DTT |
