4XKI

Human Fibroblast Growth Factor - 1 (FGF-1) mutant S116R

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	75.624, 96.274, 109.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.155 - 2.000
R-factor	0.1775
Rwork	0.175
R-free	0.21520
RMSD bond length	0.007
RMSD bond angle	1.069
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	27441
<I/σ(I)>	44.5
Completeness [%]	99.9
Redundancy	14.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	2M ammonium sulfate, 2% v/v Polyethylene glycol 400 and 0.1 M sodium HEPES pH 7.5