4XKI
Human Fibroblast Growth Factor - 1 (FGF-1) mutant S116R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-11 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 75.624, 96.274, 109.525 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.155 - 2.000 |
R-factor | 0.1775 |
Rwork | 0.175 |
R-free | 0.21520 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.069 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 27441 |
<I/σ(I)> | 44.5 |
Completeness [%] | 99.9 |
Redundancy | 14.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2M ammonium sulfate, 2% v/v Polyethylene glycol 400 and 0.1 M sodium HEPES pH 7.5 |