4XDO
Crystal structure of human KDM4C catalytic domain with OGA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 93.930, 89.770, 98.460 |
Unit cell angles | 90.00, 96.49, 90.00 |
Refinement procedure
Resolution | 40.580 - 1.970 |
R-factor | 0.2032 |
Rwork | 0.200 |
R-free | 0.25400 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.900 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.29) |
Phasing software | REFMAC |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.800 | 40.800 | 2.020 |
High resolution limit [Å] | 1.970 | 8.810 | 1.970 |
Rmerge | 0.098 | 0.037 | 0.604 |
Rpim | 0.054 | 0.022 | 0.366 |
Total number of observations | 240106 | 2528 | 15383 |
Number of reflections | 57310 | ||
<I/σ(I)> | 12 | 34.7 | 2.2 |
Completeness [%] | 99.9 | 97.6 | 99.8 |
Redundancy | 4.2 | 3.9 | 3.7 |
CC(1/2) | 0.996 | 0.998 | 0.696 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291.15 | 0.8 M Lithium chloride, 0.1 M Tris pH 8.5, 28 % w/v PEG 4000 |