4XCU
Crystal Structure of FGFR4 with an Irreversible Inhibitor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9394 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.100, 58.110, 58.670 |
Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
Resolution | 19.510 - 1.710 |
R-factor | 0.1956 |
Rwork | 0.193 |
R-free | 0.24270 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.672 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | REFMAC |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.800 |
High resolution limit [Å] | 1.710 | 1.710 |
Number of reflections | 28521 | |
<I/σ(I)> | 4.6 | 1.7 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 4.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 16% PEG 3350, 0.2M lithium sulfate, 0.1M bis-tris pH 4.5 |