4XAR

mGluR2 ECD and mGluR3 ECD complex with ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-28
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 32 2 1
Unit cell lengths	80.338, 80.338, 155.688
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.680 - 2.260
R-factor	0.1979
Rwork	0.197
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.070
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER-TNT (BUSTER 2.11.5)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.680
High resolution limit [Å]	2.260
Number of reflections	27749
<I/σ(I)>	8.2
Completeness [%]	99.0
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		295	20% PEG 3350, 200mM Ammonium Iodide