4XA5
Crystal structure of the pre-catalytic ternary complex of DNA polymerase lambda with a templating A and an incoming 8-oxo-dGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.967, 62.340, 141.613 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.790 - 1.900 |
| R-factor | 0.2004 |
| Rwork | 0.199 |
| R-free | 0.22725 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.985 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.800 | 1.904 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.800 | |
| Number of reflections | 40080 | |
| <I/σ(I)> | 22.3 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.1 M sodium cacodylate trihydrate pH 7.4 and 1.9 M sodium acetate trihydrate |
