4X6Z
Yeast 20S proteasome in complex with PR-VI modulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.350, 300.790, 144.580 |
| Unit cell angles | 90.00, 112.11, 90.00 |
Refinement procedure
| Resolution | 49.460 - 2.700 |
| R-factor | 0.17957 |
| Rwork | 0.177 |
| R-free | 0.22192 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.684 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.460 | 2.720 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 207816 | |
| <I/σ(I)> | 6.5 | 2.9 |
| Completeness [%] | 68.4 | 20.2 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 30mM magnesium acetate, 12% MPD, 0.1M MES pH 7.2 |
