4X67
Crystal structure of elongating yeast RNA polymerase II stalled at oxidative Cyclopurine DNA lesions.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 167.146, 220.798, 193.919 |
Unit cell angles | 90.00, 100.25, 90.00 |
Refinement procedure
Resolution | 50.000 - 4.100 |
R-factor | 0.2447 |
Rwork | 0.242 |
R-free | 0.28920 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.443 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.2) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 4.170 |
High resolution limit [Å] | 4.100 | 11.090 | 4.100 |
Rmerge | 0.222 | 0.035 | 0.737 |
Rmeas | 0.270 | 0.042 | 0.928 |
Rpim | 0.152 | 0.023 | 0.555 |
Total number of observations | 127175 | ||
Number of reflections | 46515 | ||
<I/σ(I)> | 3.6 | ||
Completeness [%] | 86.0 | 90 | 72.9 |
Redundancy | 2.7 | 3.2 | 2.2 |
CC(1/2) | 0.996 | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 390 mM (NH4)2HPO4/NaH2PO4, PH 5.9-6.3, 50 mM dioxane, 10 mM DTT, and 10.7% - 11.6% PEG6000 |