4X67

Crystal structure of elongating yeast RNA polymerase II stalled at oxidative Cyclopurine DNA lesions.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976
Spacegroup name	C 1 2 1
Unit cell lengths	167.146, 220.798, 193.919
Unit cell angles	90.00, 100.25, 90.00

Refinement procedure

Resolution	50.000 - 4.100
R-factor	0.2447
Rwork	0.242
R-free	0.28920
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.443
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.2)
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.170
High resolution limit [Å]	4.100	11.090	4.100
Rmerge	0.222	0.035	0.737
Rmeas	0.270	0.042	0.928
Rpim	0.152	0.023	0.555
Total number of observations	127175
Number of reflections	46515
<I/σ(I)>	3.6
Completeness [%]	86.0	90	72.9
Redundancy	2.7	3.2	2.2
CC(1/2)		0.996	0.526

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	390 mM (NH4)2HPO4/NaH2PO4, PH 5.9-6.3, 50 mM dioxane, 10 mM DTT, and 10.7% - 11.6% PEG6000