4X48
Crystal structure of GluR2 ligand-binding core
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.759, 164.965, 47.363 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.670 - 1.890 |
R-factor | 0.1747 |
Rwork | 0.173 |
R-free | 0.19720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lz5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.030 |
Data scaling software | SCALA (3.3.9) |
Phasing software | BUSTER-TNT |
Refinement software | BUSTER-TNT |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 164.965 | 164.965 | 1.990 |
High resolution limit [Å] | 1.886 | 5.960 | 1.890 |
Rmerge | 0.052 | 0.392 | |
Rmeas | 0.088 | ||
Rpim | 0.035 | 0.024 | 0.293 |
Total number of observations | 372534 | 14487 | 16680 |
Number of reflections | 69522 | ||
<I/σ(I)> | 13.7 | 26.7 | 2 |
Completeness [%] | 94.8 | 93.4 | 71.9 |
Redundancy | 5.4 | 6.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | The protein solution contained 7 mg/ml GluR2-S1S2J, 10mM (S)-Glu, 10mM HEPES (pH 7.5), 20mM NaCl and 1mM EDTA. Compound was added to a final concentration of 150micromolar from a 30mM DMSO stock. To this solution, an equal amount of reservoir solution containing 10% PEG 8000, 0.1 M ZnAc and 0.1M NaAc, pH 5.5 was added, and 2microlitre drops of this mixture were allowed to equilibrate by vapor diffusion over a 1mL reservoir. Crystals appeared in the drops in 3-5 days. |