4X42
Crystal structure of DEN4 ED3 mutant with epitope two residues substituted from DEN3 ED3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-02-07 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9800 |
| Spacegroup name | P 65 |
| Unit cell lengths | 124.585, 124.585, 86.132 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.030 - 2.780 |
| R-factor | 0.23584 |
| Rwork | 0.234 |
| R-free | 0.27277 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3we1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.512 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.830 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.135 | 0.872 |
| Number of reflections | 19294 | |
| <I/σ(I)> | 37.8 | 5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | PEG 3350, Ammonium sulphate, Tris-HCL, Dioxane |
